1PF5
Structural Genomics, Protein YJGH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-01 |
Detector | SBC-2 |
Wavelength(s) | 0.9793 |
Spacegroup name | I 21 3 |
Unit cell lengths | 127.737, 127.737, 127.737 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.160 - 2.500 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.21800 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | d*TREK |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.092 | 0.872 |
Number of reflections | 11955 | |
<I/σ(I)> | 22.04 | 1.68 |
Completeness [%] | 98.2 | 99.5 |
Redundancy | 8.31 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.2 M Lithium sulphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |