1PF3
Crystal Structure of the M441L mutant of the multicopper oxidase CueO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.445, 91.193, 53.905 |
| Unit cell angles | 90.00, 102.35, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.19283 |
| Rwork | 0.191 |
| R-free | 0.22757 |
| Starting model (for MR) | 1kv7 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.571 |
| Data scaling software | CrystalClear (D*TREK (MSC/RIGAKU)) |
| Refinement software | REFMAC (5.1.22) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 63714 | |
| <I/σ(I)> | 20.2 | 2.9 |
| Completeness [%] | 86.0 | 94 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 20% PEG, 200 mM ammonium acetate, 100 mM sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 22K |
