1PF3
Crystal Structure of the M441L mutant of the multicopper oxidase CueO
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.445, 91.193, 53.905 |
Unit cell angles | 90.00, 102.35, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.500 |
R-factor | 0.19283 |
Rwork | 0.191 |
R-free | 0.22757 |
Starting model (for MR) | 1kv7 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.571 |
Data scaling software | CrystalClear (D*TREK (MSC/RIGAKU)) |
Refinement software | REFMAC (5.1.22) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 63714 | |
<I/σ(I)> | 20.2 | 2.9 |
Completeness [%] | 86.0 | 94 |
Redundancy | 2.3 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 20% PEG, 200 mM ammonium acetate, 100 mM sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 22K |