1PEQ
Ribonucleotide Reductase Protein R1E from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9393 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 99.144, 99.144, 289.544 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.800 |
R-factor | 0.2166 |
Rwork | 0.215 |
R-free | 0.24640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | poly-alanin model of ribonucleotide reductase protein R1 from E.coli |
RMSD bond length | 0.023 |
RMSD bond angle | 1.931 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.079 | 0.286 |
Number of reflections | 36371 | |
<I/σ(I)> | 18.3 | 5.5 |
Completeness [%] | 99.1 | 99.1 |
Redundancy | 7.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 288 | sodium malonate, magnesium chloride, DTT, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 288K |