1PEO
Ribonucleotide Reductase Protein R1E from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9393 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 98.938, 98.938, 289.151 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.000 |
R-factor | 0.20847 |
Rwork | 0.206 |
R-free | 0.25240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | poly-alanin model of ribonucleotide reductase protein R1 from E.coli |
RMSD bond length | 0.029 |
RMSD bond angle | 2.359 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.110 | 0.450 |
Number of reflections | 28924 | |
<I/σ(I)> | 17.6 | 4.1 |
Completeness [%] | 99.8 | 98.4 |
Redundancy | 10.35 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 288 | sodium malonate, magnesium chloride, DTT, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 288K |