1PEE
Crystal Structure of Nitrophorin 2 complex with imidazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.827 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 34.680, 34.680, 258.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.000 - 1.500 |
| R-factor | 0.18831 |
| Rwork | 0.186 |
| R-free | 0.23980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1euo |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.917 |
| Data scaling software | d*TREK |
| Phasing software | MOLREP (V. 7.3) |
| Refinement software | REFMAC (5.1.22) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.054 | 0.125 |
| Number of reflections | 19111 | |
| <I/σ(I)> | 26.2 | 1 |
| Completeness [%] | 74.0 | 43.4 |
| Redundancy | 4.1 | 1.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.6 M sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
