1PEA

AMIDE RECEPTOR/NEGATIVE REGULATOR OF THE AMIDASE OPERON OF PSEUDOMONAS AERUGINOSA (AMIC) COMPLEXED WITH ACETAMIDE

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	0.882
Spacegroup name	P 42 21 2
Unit cell lengths	104.960, 104.960, 65.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.206
Rwork	0.206
R-free	0.27500
RMSD bond length	0.019 *
RMSD bond angle	2.200 *
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.100
Rmerge	0.101 *
Number of reflections	21687
Completeness [%]	96.0
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *		macro-seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	AmiC	12 (mg/ml)
2	1	drop	PEG4000	6 (%(w/v))
3	1	drop	Tris-HCl	20 (mM)
4	1	drop	ammonium sulfate	150 (mM)