1PDZ
X-RAY STRUCTURE AND CATALYTIC MECHANISM OF LOBSTER ENOLASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Detector technology | IMAGE PLATE |
Collection date | 1992-02 |
Wavelength(s) | 0.91 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 110.800, 110.800, 73.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 * - 2.200 |
R-factor | 0.217 * |
Rwork | 0.215 |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.600 * |
Data reduction software | MOSFLM |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 2.200 | 2.200 * |
Rmerge | 0.077 | 0.393 * |
Total number of observations | 97010 * | |
Number of reflections | 27950 | |
Completeness [%] | 91.4 | 43.1 * |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.6 * | 16 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | HEPES | 50 (mM) | |
2 | 1 | reservoir | ammonium sulfate | 2.5-2.7 (M) |