1PDH
CRYSTAL STRUCTURE OF P-HYDROXYBENZOATE HYDROXYLASE RECONSTITUTED WITH THE MODIFIED FAD PRESENT IN ALCOHOL OXIDASE FROM METHYLOTROPHIC YEASTS: EVIDENCE FOR AN ARABINOFLAVIN
Experimental procedure
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.100, 146.400, 88.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 2.100 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.069 * |
| Total number of observations | 102196 * |
| Number of reflections | 26934 * |
| Completeness [%] | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 10 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 50 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 50 (%sat) | |
| 4 | 1 | reservoir | FAD | 0.04 (mM) | |
| 5 | 1 | reservoir | EDTA | 0.15 (mM) | |
| 6 | 1 | reservoir | reduced glutathione | 0.1 (mM) | |
| 7 | 1 | reservoir | 4-hydroxybenzoate | 1 (mM) | |
| 8 | 1 | reservoir | sodium sulfite | 60 (mM) | |
| 9 | 1 | reservoir | potassium phosphate | 50 (mM) |
