1PD3
Influenza A NEP M1-binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-30 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 82.691, 82.691, 61.111 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.880 - 2.600 |
R-factor | 0.179 |
Rwork | 0.176 |
R-free | 0.24400 |
Structure solution method | MIR |
RMSD bond length | 0.043 |
RMSD bond angle | 3.300 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.600 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.090 | 0.320 |
Number of reflections | 7302 | |
<I/σ(I)> | 6 | 2 |
Completeness [%] | 99.6 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PROTEIN IN 20 MM TRIS-HCL PH7.5, 150 MM NACL, HANGING DROP METHOD, ROOM TEMPERATURE, vapor diffusion, hanging drop, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | ammonium di-phosphate | 0.4 (M) |