1PD3

Influenza A NEP M1-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-30
Detector	ADSC QUANTUM 4
Spacegroup name	P 32 2 1
Unit cell lengths	82.691, 82.691, 61.111
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.880 - 2.600
R-factor	0.179
Rwork	0.176
R-free	0.24400
Structure solution method	MIR
RMSD bond length	0.043
RMSD bond angle	3.300 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.600	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.090	0.320
Number of reflections	7302
<I/σ(I)>	6	2
Completeness [%]	99.6	100
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PROTEIN IN 20 MM TRIS-HCL PH7.5, 150 MM NACL, HANGING DROP METHOD, ROOM TEMPERATURE, vapor diffusion, hanging drop, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	ammonium di-phosphate	0.4 (M)