1PD0
Crystal structure of the COPII coat subunit, Sec24, complexed with a peptide from the SNARE protein Sed5 (yeast syntaxin-5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-04-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 94.527, 94.527, 197.935 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 * - 2.600 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.25800 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 24.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.037 | 0.195 |
Total number of observations | 73768 * | |
Number of reflections | 30516 | 3001 * |
<I/σ(I)> | 30.5 | 6.6 |
Completeness [%] | 94.5 | 94.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 * | 4 * | sodium acetate 1,6-hexanediol, cobalt chloride, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 50 (mg/ml) | |
2 | 1 | drop | 200 (mM) | ||
3 | 1 | drop | HEPES | 20 (mM) | pH7.3 |
4 | 1 | drop | dithiothreitol | 4 (mM) | |
5 | 1 | reservoir | 100 (mM) | pH5.8 | |
6 | 1 | reservoir | 1,6-hexanediol | 0.35 (M) | |
7 | 1 | reservoir | 4 (mM) |