1PCV
Crystal structure of osmotin, a plant antifungal protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Wavelength(s) | 1.100 |
Spacegroup name | P 1 |
Unit cell lengths | 41.778, 41.787, 59.559 |
Unit cell angles | 100.84, 92.41, 96.57 |
Refinement procedure
Resolution | 50.000 * - 2.300 |
Rwork | 0.211 |
R-free | 0.25820 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aun |
RMSD bond length | 0.008 |
RMSD bond angle | 1.538 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.074 * |
Total number of observations | 26588 * |
Number of reflections | 14549 * |
Completeness [%] | 83.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 22 * | lithium sulfate, nickel chloride, tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 25 (mM) | pH7.5 |
2 | 1 | drop | protein | 7 (mg/ml) | |
3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
4 | 1 | reservoir | lithium sulfate | 0.4 (M) | |
5 | 1 | reservoir | nickel chloride | 0.06 (M) |