1PCQ

Crystal structure of groEL-groES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Detector	SBC
Spacegroup name	P 21 21 2
Unit cell lengths	255.546, 266.855, 187.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.800 *
Rwork	0.262
R-free	0.27800 *
RMSD bond length	0.013 *
RMSD bond angle	1.362 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (refmac_5.1.24 24/04/2001)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800	2.800 *
Rmerge	0.138 *	0.601 *
Number of reflections	278877 *
Completeness [%]	90.3 *	55 *
Redundancy	4.0 *	3.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *	18 *	used to seeding, Xu, Z., (1997) Nature, 388, 741. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3000	12 (%)
2	1	reservoir	sodium glutamate	0.25 (M)
3	1	reservoir	cacodylate	100 (mM)
4	1	drop	PEG3000	6 (%)
5	1	drop	sodium glutamate	0.125 (M)
6	1	drop	cacodylate	50 (mM)
7	1	drop	protein	7.5 (mg/ml)