1PCF
HUMAN TRANSCRIPTIONAL COACTIVATOR PC4 C-TERMINAL DOMAIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.283, 67.814, 67.170 |
| Unit cell angles | 87.69, 84.37, 85.79 |
Refinement procedure
| Resolution | 20.000 - 1.740 |
| R-factor | 0.197 * |
| Rwork | 0.197 |
| R-free | 0.23300 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CCP4 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.780 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.060 | 0.229 |
| Total number of observations | 177425 * | |
| Number of reflections | 69529 | |
| <I/σ(I)> | 11.6 | 2.6 |
| Completeness [%] | 93.4 | 83.4 |
| Redundancy | 2.6 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 * | PROTEIN WAS CRYSTALLIZED FROM 25% MPD, 200 MM NACL AND 100 MM NAAC BUFFER (PH 4.6) |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 2 | MPD | 25 (%(v/v)) | |
| 2 | 1 | 2 | 0.2 (M) | ||
| 3 | 1 | 2 | sodium acetate | 0.1 (M) | |
| 4 | 1 | 1 | protein | 7-10 (mg/ml) | |
| 5 | 1 | 1 | HEPES | 10 (mM) | |
| 6 | 1 | 1 | 0.2 (M) |
