1PC8
Crystal Structure of a novel form of mistletoe lectin from Himalayan Viscum album L. at 3.8A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-03-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 109.182, 109.182, 309.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.800 |
| R-factor | 0.23074 |
| Rwork | 0.228 |
| R-free | 0.27106 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ce7 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.338 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.440 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 11420 | |
| <I/σ(I)> | 6.1 | 2.1 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | Ammonium Sulphate, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
