1PC5
Crystal Structure of the P50G Mutant of Ferredoxin I at 1.8 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-07-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5408 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 55.166, 55.166, 91.862 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.1826 |
Rwork | 0.180 |
R-free | 0.25300 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.040 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 13643 | |
Completeness [%] | 92.2 | 93.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.4 | 2 * | Shen, B., (1993) J.Biol.Chem., 268, 25928., Stout, C.D., (1979) J.Biol.Chem., 254, 3598. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7-10 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 0.5 (M) | |
3 | 1 | drop | ammonium sulfate | 1.2 (M) | |
4 | 1 | reservoir | ammonium sulfate | 3.5 (M) |