1PC3
Crystal structure of the extracellular phosphate ABC transport receptor (PstS-1) and immunodominant antigen of M. tuberculosis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 281 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-09-08 |
| Detector | SDMS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 125.400, 72.300, 73.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.640 - 2.160 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.26400 |
| Structure solution method | MIR |
| Starting model (for MR) | PDB id 2ABH |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | STRATEGY |
| Data scaling software | SDMS |
| Phasing software | PHASES |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.640 | 2.300 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.065 | 0.084 |
| Number of reflections | 32319 | |
| <I/σ(I)> | 8 | 2.64 |
| Completeness [%] | 85.5 | 79.4 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | PEG 4000, ammonium acetate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
