1PBV

SEC7 DOMAIN OF THE EXCHANGE FACTOR ARNO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE
Synchrotron site	LURE
Detector	MARRESEARCH
Spacegroup name	P 21 3
Unit cell lengths	89.766, 89.766, 89.766
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.000
R-factor	0.182
Rwork	0.182
R-free	0.22600
Structure solution method	MIRAS
RMSD bond length	0.009
RMSD bond angle	2.049
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	X-PLOR (3.84)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.000
Rmerge	0.042 *
Total number of observations	70772 *
Number of reflections	16226 *
Completeness [%]	97.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *
1	Vapor diffusion, hanging drop *	8.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	PEG4000	12.5 (%)
3	1	reservoir		200 (mM)
4	1	reservoir	propanol	3.5 (%)
5	1	reservoir	dithiothreitol	1 (mM)
6	1	reservoir	EPPS	100 (mM)