1PBT
The crystal structure of TM1154, oxidoreductase, sol/devB family from Thermotoga maritima
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-20 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97932, 0.97952, 0.946 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.729, 75.084, 148.252 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.940 - 1.700 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.22500 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.800 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.091 | 0.279 |
| Number of reflections | 22136 | |
| <I/σ(I)> | 7.7 | 3 |
| Completeness [%] | 94.1 | 64.6 |
| Redundancy | 7.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG MME 5000, ammonium sulfate, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
