1PBN
PURINE NUCLEOSIDE PHOSPHORYLASE
Experimental procedure
| Detector technology | AREA DETECTOR |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 94.200, 94.200, 94.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.27000 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.180 * |
| Data reduction software | SDMS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Rmerge | 0.130 |
| Number of reflections | 17293 |
| Completeness [%] | 80.0 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 31-35 (%) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | or Tris-HCl |
| 3 | 1 | reservoir | beta-D-glucopyranoside | 1 (%) |
