1PBG

THE THREE-DIMENSIONAL STRUCTURE OF 6-PHOSPHO-BETA GALACTOSIDASE FROM LACTOCOCCUS LACTIS

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1994
Detector	SIEMENS-NICOLET X100
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	60.500, 83.200, 105.700
Unit cell angles	90.00, 93.70, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.164
Rwork	0.164
R-free	0.23600
RMSD bond length	0.010
RMSD bond angle	24.220 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.300
Rmerge	0.069
Number of reflections	36765
Completeness [%]	78.8
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	26-30 (%)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir		0.2 (M)
4	1	reservoir		0.02 (%)