1PBG
THE THREE-DIMENSIONAL STRUCTURE OF 6-PHOSPHO-BETA GALACTOSIDASE FROM LACTOCOCCUS LACTIS
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1994 |
| Detector | SIEMENS-NICOLET X100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.500, 83.200, 105.700 |
| Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.164 |
| Rwork | 0.164 |
| R-free | 0.23600 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.220 * |
| Data reduction software | XDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.069 |
| Number of reflections | 36765 |
| Completeness [%] | 78.8 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 26-30 (%) | |
| 2 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | 0.02 (%) |
