1PBE

CRYSTAL STRUCTURE OF THE P-HYDROXYBENZOATE HYDROXYLASE-SUBSTRATE COMPLEX REFINED AT 1.9 ANGSTROMS RESOLUTION. ANALYSIS OF THE ENZYME-SUBSTRATE AND ENZYME-PRODUCT COMPLEXES

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	71.500, 145.800, 88.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.156
RMSD bond length	0.018
RMSD bond angle	0.039
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.126 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	free interface diffusion method *	7.5 *	4-20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	drop	p-hydroxybenzoate	2 (mM)
3	1	drop	FAD	0.04 (mM)
4	1	drop	EDTA	0.3 (mM)
5	1	drop	sodium sulphite	30 (mM)
6	1	drop	phosphate	0.1 (M)
7	1	reservoir	ammonium sulphate	70 (%sat)
8	1	reservoir	phosphate	0.1 (M)