1PBE
CRYSTAL STRUCTURE OF THE P-HYDROXYBENZOATE HYDROXYLASE-SUBSTRATE COMPLEX REFINED AT 1.9 ANGSTROMS RESOLUTION. ANALYSIS OF THE ENZYME-SUBSTRATE AND ENZYME-PRODUCT COMPLEXES
Experimental procedure
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.500, 145.800, 88.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.900 |
R-factor | 0.156 |
RMSD bond length | 0.018 |
RMSD bond angle | 0.039 |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.900 * |
Rmerge | 0.126 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | free interface diffusion method * | 7.5 * | 4-20 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-10 (mg/ml) | |
2 | 1 | drop | p-hydroxybenzoate | 2 (mM) | |
3 | 1 | drop | FAD | 0.04 (mM) | |
4 | 1 | drop | EDTA | 0.3 (mM) | |
5 | 1 | drop | sodium sulphite | 30 (mM) | |
6 | 1 | drop | phosphate | 0.1 (M) | |
7 | 1 | reservoir | ammonium sulphate | 70 (%sat) | |
8 | 1 | reservoir | phosphate | 0.1 (M) |