1PB0
YCDX PROTEIN IN AUTOINHIBITED STATE
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 134.130, 77.330, 82.280 |
| 格子定数 [度] | 90.00, 103.17, 90.00 |
精密化法
| 残基 | 10.000 - 1.950 |
| Rwork | 0.164 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m65 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.361 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 2.000 |
| 分解能 [Å] (高) | 1.950 | 1.950 |
| Rmerge_l_obs | 0.081 | 0.212 |
| 独立反射数 | 59787 | |
| <I/σ(I)> | 16.2 | 3.3 |
| 完全性 [%] | 99.8 | 98.6 |
| 冗長性 | 4.7 | 4.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1 M Tris, 60% AS, 3% MPD, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
