1PAR
DNA RECOGNITION BY BETA-SHEETS IN THE ARC REPRESSOR-OPERATOR CRYSTAL STRUCTURE
Experimental procedure
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.670, 56.730, 53.800 |
Unit cell angles | 90.00, 106.90, 90.00 |
Refinement procedure
Resolution | 21.000 - 2.600 |
R-factor | 0.225 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.510 |
Refinement software | TNT |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.600 |
Rmerge | 0.114 * |
Total number of observations | 161619 * |
Number of reflections | 11253 |
Completeness [%] | 95.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | pH 7.40, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | BIS-TRIS-PROPANE | ||
3 | 1 | 1 | NA AZIDE | ||
4 | 1 | 2 | WATER | ||
5 | 1 | 2 | PEG 400 | ||
6 | 1 | 2 | MGCL2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Arc tetramer | 0.58 (mM) | |
2 | 1 | drop | DNA duplex | 0.93 (mM) | |
3 | 1 | drop | Bis-Tris-propane-Cl | 30 (mM) | pH7.4 |
4 | 1 | drop | 1 (mM) | ||
5 | 1 | reservoir | PEG400 | 15 (%) | |
6 | 1 | reservoir | 100 (mM) |