1PAQ
CRYSTAL STRUCTURE OF THE CATALYTIC FRAGMENT OF EUKARYOTIC INITIATION FACTOR 2B EPSILON
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9821, 0.9824, 0.9121 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 106.340, 106.340, 91.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.2439 |
| Rwork | 0.243 |
| R-free | 0.27200 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 * | 0.278 * |
| Number of reflections | 22162 | |
| Completeness [%] | 99.2 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 * | 5 * | PEG 2000MME, ammonium acetate, Tris-HCl, pH 5.75, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | pH7.4 |
| 2 | 1 | drop | dithiothreitol | 5 (mM) | |
| 3 | 1 | drop | PMSF | 1 (mM) | |
| 4 | 1 | drop | EDTA | 0.5 (mM) | |
| 5 | 1 | drop | 100 (mM) | ||
| 6 | 1 | drop | protein | 5-8 (mg/ml) | |
| 7 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 8 | 1 | reservoir | PEG2000 MME | 23-25 (%) | |
| 9 | 1 | reservoir | sodium citrate | 100 (mM) | pH7.57 |
