1PAQ
CRYSTAL STRUCTURE OF THE CATALYTIC FRAGMENT OF EUKARYOTIC INITIATION FACTOR 2B EPSILON
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9821, 0.9824, 0.9121 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 106.340, 106.340, 91.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.2439 |
Rwork | 0.243 |
R-free | 0.27200 * |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.050 * | 0.278 * |
Number of reflections | 22162 | |
Completeness [%] | 99.2 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.4 * | 5 * | PEG 2000MME, ammonium acetate, Tris-HCl, pH 5.75, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 20 (mM) | pH7.4 |
2 | 1 | drop | dithiothreitol | 5 (mM) | |
3 | 1 | drop | PMSF | 1 (mM) | |
4 | 1 | drop | EDTA | 0.5 (mM) | |
5 | 1 | drop | 100 (mM) | ||
6 | 1 | drop | protein | 5-8 (mg/ml) | |
7 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
8 | 1 | reservoir | PEG2000 MME | 23-25 (%) | |
9 | 1 | reservoir | sodium citrate | 100 (mM) | pH7.57 |