Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

1PA7

Crystal structure of amino-terminal microtubule binding domain of EB1

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X8C
Synchrotron siteNSLS
BeamlineX8C
Temperature [K]100
Detector technologyCCD
Collection date2002-10-07
DetectorADSC QUANTUM 4
Wavelength(s)0.9800
Spacegroup nameP 1 21 1
Unit cell lengths31.856, 48.453, 44.968
Unit cell angles90.00, 103.36, 90.00
Refinement procedure
Resolution20.000 - 1.450
R-factor0.17358
Rwork0.173
R-free0.18900

*

Structure solution methodMAD
RMSD bond length0.022

*

RMSD bond angle1.250

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareDM
Refinement softwareREFMAC (5.1.19)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0001.500
High resolution limit [Å]1.4501.450
Rmerge0.0420.192

*

Number of reflections23798
Completeness [%]99.0

*

97.8

*

Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP8

*

298PEG4K, ammonium sulfate, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropTris10 (mM)pH8.0
21drop0.1 (M)
31dropdithiothreitol1 (mM)
41dropprotein15 (mg/ml)
51reservoirPEG335020 (%)
61reservoirammonium sulfate0.2 (M)
71reservoirMES0.1 (M)pH6.0

217705

PDB entries from 2024-03-27

PDB statisticsPDBj update infoContact PDBjnumon