1PA7
Crystal structure of amino-terminal microtubule binding domain of EB1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-10-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9800 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.856, 48.453, 44.968 |
Unit cell angles | 90.00, 103.36, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.450 |
R-factor | 0.17358 |
Rwork | 0.173 |
R-free | 0.18900 * |
Structure solution method | MAD |
RMSD bond length | 0.022 * |
RMSD bond angle | 1.250 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | DM |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.042 | 0.192 * |
Number of reflections | 23798 | |
Completeness [%] | 99.0 * | 97.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 298 | PEG4K, ammonium sulfate, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris | 10 (mM) | pH8.0 |
2 | 1 | drop | 0.1 (M) | ||
3 | 1 | drop | dithiothreitol | 1 (mM) | |
4 | 1 | drop | protein | 15 (mg/ml) | |
5 | 1 | reservoir | PEG3350 | 20 (%) | |
6 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
7 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |