1PA6
Crystal structure of the OXYTRICHA nova telomere end-binding protein complexed with noncognate ssDNA GGGGTTTTGAGG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 93.110, 93.110, 422.680 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.790 - 2.450 |
R-factor | 0.264 |
Rwork | 0.223 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 24.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.790 |
High resolution limit [Å] | 2.450 |
Number of reflections | 31008 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | Horvath, M.P., (1998) Cell., 95, 963. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | ethylene glycol | 30 (%) | |
2 | 1 | drop | PEG4000 | 1-6 (%) | |
3 | 1 | drop | MES | 40 (mM) | pH5.5-7.0 |
4 | 1 | drop | 0.02 (%) | ||
5 | 1 | drop | dithiothreitol | 2 (mM) | |
6 | 1 | reservoir | 50 (mM) |