1P9Y
Ribosome binding of E. coli Trigger Factor mutant F44L.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-13 |
Detector | MARRESEARCH |
Wavelength(s) | 1.12 |
Spacegroup name | H 3 2 |
Unit cell lengths | 71.901, 71.901, 257.659 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 * - 2.150 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oms |
RMSD bond length | 0.007 |
RMSD bond angle | 22.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.078 | 0.201 |
Number of reflections | 14381 | |
<I/σ(I)> | 25.2 | 6.5 |
Completeness [%] | 99.7 | 96.7 |
Redundancy | 11.3 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 * | 6 * | PEG 2000 MME, AMMONIUM SULFATE, SODIUM ACETATE, TRIS, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 1.5 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |
4 | 1 | reservoir | PEG400 | 5 (%) | |
5 | 1 | reservoir | glycerol | 5 (%) | |
6 | 1 | reservoir | PEG2000 MME | 31 (%) |