1P9R
Crystal Structure of Vibrio cholerae putative NTPase EpsE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9748 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 103.611, 103.611, 166.261 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.21653 |
| Rwork | 0.214 |
| R-free | 0.26500 * |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.374 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE (V. 2.02) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.102 * | |
| Total number of observations | 241296 * | |
| Number of reflections | 18982 | |
| <I/σ(I)> | 18.6 | 4.3 |
| Completeness [%] | 100.0 | 65.3 * |
| Redundancy | 12.71 * | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 * | 293 | PEG 200,3-morpholinopropanesulfonate, ammonium acetate, AMP-PNP, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | TEA | 0.1 (M) | |
| 3 | 1 | drop | 0.5 (M) | ||
| 4 | 1 | drop | glycerol | 10 (%) | |
| 5 | 1 | drop | TCEP | 1 (mM) | |
| 6 | 1 | drop | EDTA | 1 (mM) | |
| 7 | 1 | reservoir | PEG5000 MME | 12-18 (%(v/v)) | |
| 8 | 1 | reservoir | ammonium sulfate | 0.15-0.20 (M) | |
| 9 | 1 | reservoir | MES | 0.1 (M) | pH6.3 |
