1P9N
Crystal structure of Escherichia coli MobB.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97936 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 239.001, 239.001, 49.046 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 * - 2.800 |
R-factor | 0.275 |
Rwork | 0.275 |
R-free | 0.30800 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 24.300 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.700 |
High resolution limit [Å] | 2.800 * | 2.610 |
Rmerge | 0.183 * | 0.716 * |
Total number of observations | 241509 * | |
Number of reflections | 20053 * | |
Completeness [%] | 98.5 * | 87.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 295 | 1.68 M ammonium sulphate, 50 mM Malonic acid, 15 mM cetyl ammonium bromide, 10 mM MgCl2, 1 mM B-mercaptoethanol., pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 22K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.0 (mg/ml) | |
10 | 1 | reservoir | beta-mercaptoethanol | 1 (mM) | |
2 | 1 | drop | Tris | 50 (mM) | pH7.5 |
3 | 1 | drop | 200 (mM) | ||
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | drop | glycerol | 5 (%(v/v)) | |
6 | 1 | reservoir | ammonium sulfate | 1.68 (M) | |
7 | 1 | reservoir | malonic acid | 0.5 (M) | pH4.5 |
8 | 1 | reservoir | acetyl ammonium bromide | 15 (mM) | |
9 | 1 | reservoir | 10 (mM) |