1P9I

Coiled-coil X-ray structure at 1.17 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	C 2 2 21
Unit cell lengths	18.098, 117.287, 22.209
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.170
Rwork	0.179
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009 *
RMSD bond angle	1.800 *

Data quality characteristics

	Overall
Low resolution limit [Å]	20.770
High resolution limit [Å]	1.170
Rmerge	0.037 *
Total number of observations	263880 *
Number of reflections	8183 *
Completeness [%]	97.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium acetate	0.1-0.2 (M)
2	1	reservoir	PEG4000	30 (%)
3	1	reservoir	sodium acetate	0.1 (M)	pH4.6
4	1	drop	protein	20 (mg/ml)