1P9G
Crystal structure of a novel antifungal protein distinct with five disulfide bridges from Ecommia ulmoides Oliver at atomic resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 283 |
| Detector technology | CCD |
| Collection date | 2002-03-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 19.011, 23.178, 30.720 |
| Unit cell angles | 90.00, 98.56, 90.00 |
Refinement procedure
| Resolution | 100.000 - 0.840 |
| R-factor | 0.0684 |
| Rwork | 0.068 |
| R-free | 0.07940 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.035 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 0.870 |
| High resolution limit [Å] | 0.840 | 0.840 |
| Rmerge | 0.051 | 0.140 |
| Number of reflections | 22113 | |
| <I/σ(I)> | 26.6 | 8.5 |
| Completeness [%] | 86.8 | 70.4 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | SODIUM ACETATE, ISO-PROPANOL, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
