1P9A
Crystal Structure of N-Terminal Domain of Human Platelet Receptor Glycoprotein Ib-alpha at 1.7 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0642 |
Spacegroup name | P 43 |
Unit cell lengths | 51.734, 51.734, 114.041 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.700 |
R-factor | 0.205 |
Rwork | 0.204 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2.7 Angstrom P21/GPIb-alpha MAD structure-to be published |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 160737 | |
<I/σ(I)> | 19.8 | 5.3 |
Completeness [%] | 95.4 | 73.6 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 4 * | PEG 6000, Sodium Nitrate, Sodium Acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 2.5 (mM) | |
2 | 1 | drop | 50 (mM) | pH7.4 | |
3 | 1 | drop | protein | 15-20 (mg/ml) | |
4 | 1 | reservoir | PEG6000 | 12 (%(w/w)) | |
5 | 1 | reservoir | sodium nitrate | 200 (mM) | |
6 | 1 | reservoir | sodium acetate | 100 (mM) | pH5.6 |