1P92

Crystal Structure of (H79A)DtxR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	2001-11-05
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	63.390, 63.390, 109.872
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.450 - 2.100
R-factor	0.243
Rwork	0.243
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1BYM 2tdx
RMSD bond length	0.007
RMSD bond angle	2.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.100
Rmerge	0.078
Number of reflections	15278
<I/σ(I)>	12.9
Completeness [%]	98.8
Redundancy	2.24

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 400, ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K