1P91
Crystal Structure of RlmA(I) enzyme: 23S rRNA n1-G745 methyltransferase (NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ER19)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2002-10-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97889, 0.97874, 0.9500 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 107.187, 122.279, 143.138 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.29600 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.270 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.106 | |
Total number of observations | 88268 * | |
Number of reflections | 21876 | |
<I/σ(I)> | 11 | |
Completeness [%] | 93.0 | 80.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 300 | Ammonium sulfate, Lithium Sulfate, pH 6.5, VAPOR DIFFUSION, temperature 300K |
1 | Vapor diffusion, hanging drop * | 7.5 * | 300 | Ammonium sulfate, Lithium Sulfate, pH 6.5, VAPOR DIFFUSION, temperature 300K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
3 | 1 | drop | SAM | 5 (mM) | |
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 0.5 (M) | |
6 | 1 | reservoir | lithium sulfate | 1.0 (M) | |
7 | 1 | reservoir | sodium citrate | 0.1 (M) | pH6.5 |