1P8U

BACTERIORHODOPSIN N' INTERMEDIATE AT 1.62 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 63
Unit cell lengths	60.981, 60.981, 108.312
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 1.620
R-factor	0.1619
R-free	0.20600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m0l
RMSD bond length	0.015
RMSD bond angle	0.048 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.680
High resolution limit [Å]	1.620	1.620
Rmerge	0.050	0.668
Total number of observations	469682 *
Number of reflections	28071
<I/σ(I)>	35.7	3.6
Completeness [%]	96.8	95.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.6	295	Gabriele, R., (1998) J. Struct. Biol., 121, 82. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	3.3 (mg/ml)
2	1	1	sodium potassium Pi	1 (M)
3	1	1	MPD	2.5 (%)