1P8T
Crystal structure of Nogo-66 Receptor
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.21 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 123.958, 123.958, 120.173 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 * - 3.200 |
| R-factor | 0.266 |
| Rwork | 0.265 |
| R-free | 0.29200 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 * |
| RMSD bond angle | 2.270 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | |
| High resolution limit [Å] | 3.200 | |
| Rmerge | 0.071 * | |
| Number of reflections | 34022 | |
| Completeness [%] | 99.6 | 99 * |
| Redundancy | 4.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.2 * | 293 | NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 250 (mM) | ||
| 3 | 1 | drop | HEPES | 10 (mM) | pH7.2 |
| 4 | 1 | reservoir | 3.7 (M) | ||
| 5 | 1 | reservoir | MES | 100 (mM) | pH6.5 |
