1P8H

BACTERIORHODOPSIN M1 INTERMEDIATE PRODUCED AT ROOM TEMPERATURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-27
Detector	ADSC QUANTUM
Wavelength(s)	0.979
Spacegroup name	P 63
Unit cell lengths	60.907, 60.907, 108.574
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 1.520
R-factor	0.1754
R-free	0.20700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m0l
RMSD bond length	0.015
RMSD bond angle	0.047
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.560
High resolution limit [Å]	1.520	1.520
Rmerge	0.043	0.620
Total number of observations	646932 *
Number of reflections	36506
<I/σ(I)>	41.5	3.7
Completeness [%]	92.1	80.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.6	295	Gabriele, R., (1998) J. Struct. Biol., 121, 82. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	3.3 (mg/ml)
2	1	1	sodium potassium Pi	1 (M)
3	1	1	MPD	2.5 (%)