1P7S

T4 LYSOZYME CORE REPACKING MUTANT V103I/TA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-06-16
Detector	MARRESEARCH
Wavelength(s)	1.23979
Spacegroup name	P 21 21 21
Unit cell lengths	30.787, 54.862, 88.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.600 - 1.500
R-factor	0.193
Rwork	0.192
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.012
RMSD bond angle	2.100 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.086	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.056	0.351
Number of reflections	23577
<I/σ(I)>	7.4	2.2
Completeness [%]	95.3	78 *
Redundancy	3.5	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *	277	2M K/Na phosphate, NaCl, BME, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	0.1 (M)	pH7.5
2	1	reservoir	PEG3400	20 (%(w/v))
3	1	reservoir	isopropanol	5 (%(v/v))