1P7D
Crystal structure of the Lambda Integrase (residues 75-356) bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-06 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.021, 106.161, 73.165 |
| Unit cell angles | 90.00, 92.10, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.950 |
| Rwork | 0.231 |
| R-free | 0.25900 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.290 |
| Data reduction software | TRUNCATE |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.070 | 0.338 |
| Number of reflections | 19010 | |
| <I/σ(I)> | 18.8 | 3.6 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 22 * | PEG 8000, Ammonium Sulfate, Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | Ammonium Sulfate | ||
| 3 | 1 | 1 | Sodium Cacodylate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium cacodylate | 100 (mM) | pH6.5 |
| 2 | 1 | drop | PEG8000 | 18-30 (%) | |
| 3 | 1 | drop | ammonium sulfate | 20-100 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 30 (%) |
