1P65

Crystal structure of the nucleocapsid protein of porcine reproductive and respiratory syndrome virus (PRRSV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-27
Detector	MARRESEARCH
Wavelength(s)	1.74
Spacegroup name	P 32 2 1
Unit cell lengths	44.657, 44.657, 125.347
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.600
R-factor	0.2124
Rwork	0.210
R-free	0.26700 *
Structure solution method	S-SAD
RMSD bond length	0.015
RMSD bond angle	1.770 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.680
High resolution limit [Å]	2.600	2.600
Rmerge	0.041	0.220
Number of reflections	4813	389 *
<I/σ(I)>	24.6	12
Completeness [%]	99.0	100
Redundancy	19.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	15 *	PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	8 (%)	pH6.5