1P65
Crystal structure of the nucleocapsid protein of porcine reproductive and respiratory syndrome virus (PRRSV)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-27 |
Detector | MARRESEARCH |
Wavelength(s) | 1.74 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 44.657, 44.657, 125.347 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.600 |
R-factor | 0.2124 |
Rwork | 0.210 |
R-free | 0.26700 * |
Structure solution method | S-SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.770 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 2.680 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.041 | 0.220 |
Number of reflections | 4813 | 389 * |
<I/σ(I)> | 24.6 | 12 |
Completeness [%] | 99.0 | 100 |
Redundancy | 19.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 15 * | PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG3350 | 8 (%) | pH6.5 |