1P5X
STRUCTURE OF THE D55N MUTANT OF PHOSPHOLIPASE C FROM BACILLUS CEREUS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2000-02-21 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 90.140, 90.140, 74.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.19700 * |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1ah7 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.111 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.053 | 0.136 |
Number of reflections | 19337 | |
<I/σ(I)> | 22 | |
Completeness [%] | 93.0 | 94.1 |
Redundancy | 3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6 | 298 | AMMOUNIUM SULPHATE, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 3.75 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 24.6 (%sat) | |
3 | 1 | reservoir | ammonium sulfate | 40 (%sat) |