1P5V
X-ray structure of the Caf1M:Caf1 chaperone:subunit preassembly complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.000, 69.230, 69.110 |
| Unit cell angles | 90.00, 92.96, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 * |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.24100 * |
| Structure solution method | MAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.077 | 0.791 |
| Number of reflections | 34122 | |
| <I/σ(I)> | 20.9 | 1.6 |
| Completeness [%] | 90.5 | 69.5 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Zavialov, A., (2003) Acta Crystallogr.,Sect.D, 59, 359. * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Zavialov, A., (2003) Acta Crystallogr.,Sect.D, 59, 359. * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Zavialov, A., (2003) Acta Crystallogr.,Sect.D, 59, 359. * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Zavialov, A., (2003) Acta Crystallogr.,Sect.D, 59, 359. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15-20 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 20-30 (%) | |
| 3 | 1 | reservoir | PEG8000 | 20-30 (%) |
