1P5C
Circular permutation of Helix A in T4 lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 170 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.758, 67.763, 94.057 |
| Unit cell angles | 90.00, 92.58, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.31000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2lzm |
| RMSD bond length | 0.012 |
| RMSD bond angle | 13.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 22893 | |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 97.4 | 99.3 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | 30% PEG 3400, 50mM Phosphate buffer, 5% isopropanol, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
