1P59
Structure of a non-covalent Endonuclease III-DNA Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-11-20 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 66.321, 105.896, 41.518 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.500 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.26900 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1orn |
RMSD bond length | 0.008 |
RMSD bond angle | 19.100 * |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.056 | 0.271 |
Number of reflections | 17421 | |
<I/σ(I)> | 19.6 | 3.7 |
Completeness [%] | 89.0 | 83.1 |
Redundancy | 3.3 * | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 8000, TRIS, BME, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8000 | ||
2 | 1 | 1 | TRIS | ||
3 | 1 | 1 | BME | ||
4 | 1 | 2 | PEG 8000 | ||
5 | 1 | 2 | TRIS | ||
6 | 1 | 2 | BME |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
2 | 1 | reservoir | PEG8000 | 20-25 (%(w/v)) | |
3 | 1 | reservoir | beta-mercaptoethanol | 5 (mM) |