1P51
Anabaena HU-DNA cocrystal structure (AHU6)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.646, 107.836, 83.464 |
Unit cell angles | 90.00, 92.25, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.500 |
R-factor | 0.291 |
Rwork | 0.286 |
R-free | 0.33800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1P71 with nonidentical sidechains pruned back to a common atom |
RMSD bond length | 0.005 |
RMSD bond angle | 0.927 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.570 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.110 * | 0.359 |
Number of reflections | 23324 | |
Completeness [%] | 93.3 | 46.5 |
Redundancy | 3 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 292 | Peg 5000 monomethyl ether, glycerol, tris, jeffamine, potassium chloride, calcium chloride, sodium azide, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Peg 5000 | ||
10 | 1 | 2 | Peg 5000 | ||
11 | 1 | 2 | glycerol | ||
12 | 1 | 2 | potassium chloride | ||
13 | 1 | 2 | calcium chloride | ||
14 | 1 | 2 | sodium azide | ||
15 | 1 | 2 | H2O | ||
2 | 1 | 1 | monomethyl ether | ||
3 | 1 | 1 | glycerol | ||
4 | 1 | 1 | tris | ||
5 | 1 | 1 | jeffamine | ||
6 | 1 | 1 | potassium chloride | ||
7 | 1 | 1 | calcium chloride | ||
8 | 1 | 1 | sodium azide | ||
9 | 1 | 1 | H2O |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 50 (mM) | ||
2 | 1 | reservoir | glycerol | 10 (%) | |
3 | 1 | reservoir | Tris | 50 (mM) | pH7.5 or pH8.0 |
4 | 1 | reservoir | 0.02 (%) | ||
5 | 1 | reservoir | Jaffamine | 1 (%) | pH7.0 |
6 | 1 | reservoir | PEG5000 MME | 12-22.5 (%) | |
7 | 1 | reservoir | 10 (mM) |