1P50
Transition state structure of an Arginine Kinase mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-03-10 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.454, 71.246, 80.116 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.800 |
Rwork | 0.172 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bg0 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.128 | |
Number of reflections | 8914 | |
Completeness [%] | 97.4 * | 92 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 4 * | PEG 6000, Hepes, magnesium chloride, ADP, nitrate, arginine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | Vapor diffusion * | 7.5 | 4 * | PEG 6000, Hepes, magnesium chloride, ADP, nitrate, arginine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | Vapor diffusion * | 7.5 | 4 * | PEG 6000, Hepes, magnesium chloride, ADP, nitrate, arginine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | Vapor diffusion * | 7.5 | 4 * | PEG 6000, Hepes, magnesium chloride, ADP, nitrate, arginine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 24 (mg/ml) | |
2 | 1 | drop | PEG6000 | 32 (%) | |
3 | 1 | drop | HEPES | 0.025 (M) | pH7.5 |
4 | 1 | drop | 0.05 (M) | ||
5 | 1 | drop | PEG6000 | 16 (%) | |
6 | 1 | reservoir | PEG6000 | 26 (%) |