1P4P
Outer Surface Protein B of B. burgdorferi: crystal structure of the C-terminal fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 283 |
Detector technology | IMAGE PLATE |
Collection date | 1997-03-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979, 1.073 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 30.800, 51.660, 158.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
Rwork | 0.211 |
R-free | 0.26030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1osp |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.063 | 0.206 |
Number of reflections | 8090 | |
<I/σ(I)> | 34.1 | 12.6 |
Completeness [%] | 89.8 | 77.9 |
Redundancy | 23.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 295 | PEG550MME, Bicine, sodium bromide, sodium citrate, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |