1P3R
Crystal structure of the phosphotyrosin binding domain(PTB) of mouse Disabled 1(Dab1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.02466 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 128.150, 128.150, 272.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| Rwork | 0.223 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m7e |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.310 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.100 | 2.300 * |
| Rmerge | 0.084 * | |
| Total number of observations | 2717217 * | |
| Number of reflections | 49672 | |
| <I/σ(I)> | 54.7 | |
| Completeness [%] | 97.1 | 99.4 * |
| Redundancy | 54.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 18 * | sodium formate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 20 (mM) | pH7.5 |
| 2 | 1 | drop | dithiothreitol | 1 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | protein | 20 (mg/ml) | |
| 5 | 1 | reservoir | sodium formate | 3.5 (M) | |
| 6 | 1 | reservoir | HEPES | 50 (mM) | pH8.5 |
