1P3C
Glutamyl endopeptidase from Bacillus intermedius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.10 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.494, 85.437, 82.147 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.320 - 1.500 |
| R-factor | 0.156 |
| Rwork | 0.153 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1agj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.430 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 33011 | |
| Completeness [%] | 97.3 | 95.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.01 M Tris-HCl buffer, pH 7.0, 2 mM CaCl2, 1.2 M potassium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
