1P3B
Crystallographic Studies of Nucleosome Core Particles containing Histone 'Sin' Mutants
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.180, 109.520, 182.441 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 3.000 |
| Rwork | 0.219 |
| R-free | 0.29000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aoi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.190 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.070 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.059 | 0.271 |
| Number of reflections | 43674 * | |
| <I/σ(I)> | 14.3 | 2.8 |
| Completeness [%] | 99.9 * | 99.8 |
| Redundancy | 4.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 20 * | Luger, K., (1997) Nature, 389, 251. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MnCl2 | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | Potassium cacodylate | ||
| 4 | 1 | 2 | MnCl2 | ||
| 5 | 1 | 2 | KCl |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | 70-75 (mM) | ||
| 4 | 1 | drop | potassium cacodylate | 20 (mM) | pH6.0 |
| 5 | 1 | reservoir | 40-46 (mM) | ||
| 6 | 1 | reservoir | 35-40 (mM) | ||
| 7 | 1 | reservoir | potassium cacodylate | 20 (mM) | pH6.0 |
